Table 2.
Comparison of five viability markers in eggs deposited in liver and colon tissue from mice infected with S. japonicum
| Staining method | Marker | Positive rate (positive/total eggs; %) at different days post infection | |||||
|---|---|---|---|---|---|---|---|
| Eggs in hepatic tissue | Eggs in colon tissue | ||||||
| 45d | 90d | 180d | 45d | 90d | 180d | ||
| Viable morphology | 90.7 (3857/4253) |
54.2* (2510/4631) |
35.0* (1511/4318) |
91.1 (4251/4666) |
53.8* (2604/4840) |
35.5* (1609/4532) |
|
| NBT/ BCIP |
ALP | 100.00 (368/368) |
82.25 (306/372 |
48.54 (234/482) |
100.00 (287/287) |
74.36 (302/406) |
66.59 (281/422) |
| von Kossa |
CalS▼ | 100.00 (460/460) |
63.86* (440/689) |
43.14 (280/649) |
100.00 (378/378) |
45.89* (179/390) |
35.87* (155/432) |
| DAB | AOS | 92.68 (2192/2365) |
54.05* (1308/2420) |
42.70 (889/2082) |
93.45 (2170/2322) |
46.03* (976/2120) |
36.28* (755/2086) |
| MTT | SDHG | 98.29 (1607/1635) |
60.42* (815/1349) |
50.52 (776/1536) |
99.19 (1348/1359) |
63.71* (741/1163) |
59.36 (631/1063) |
ALP (alkaline phosphatase), CalS (calcified substance), AOS (antioxidase) and SDHG (succinic dehydrogenase). ▼: Data are non-calcified number of eggs. *: Compared with ALP, P < 0.05. All the data were collected from examining 18 blocks of liver and intestinal tissue samples from each of six mice