Table 3.
Comparison of five markers in eggs deposited in liver and colon tissue from mice infected with S. japonicum post treatment
| Staining method |
Marker | Positive rate (positive / total eggs; %) at different days post treatment with PZQ | |||||
|---|---|---|---|---|---|---|---|
| Eggs in hepatic tissue | Eggs in colon tissue | ||||||
| 30d | 90d | 180d | 30d | 90d | 180d | ||
| Viable morphology | 2.7* (91/3369) |
0.00* (0/2713) |
0.00 (0/1548) |
2.8* (126/4497) |
0.00 (0/2320) |
0.00 (0/1587) |
|
| NBT/ BCIP |
ALP | 83.52 (304/364) |
34.88 (75/215) |
5.83 (6/103) |
75.35 (217/288) |
0.00 (0/187) |
0.00 (0/ 149) |
| von Kossa |
CalS▼ | 78.57 (176/224) |
15.22* (21/138) |
2.97 (3/101) |
68.75 (143/208) |
8.45 (12/142) |
2.94 (3/102) |
| DAB | AOS | 13.97** (96/687) |
0.00** (0/323) |
0.00 (0/312) |
19.90** (83/417) |
0.00 (0/220) |
0.00 (0/126) |
| MTT | SDHG | 11.41** (67/587) |
5.70** (29/509) |
0.00 (0/456) |
7.16** (38 /531) |
3.08 (9/292) |
0.00 (0/266) |
ALP (alkaline phosphatase), CalS (calcified substance), AOS (antioxidase) and SDHG (succinic dehydrogenase). ▼: Data ares non-calcified number of eggs. Compared with ALP: * P < 0.05; ** P < 0.01. All the data were collected from examining 18 blocks of liver or intestinal tissue samples from each of six mice