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. 2017 Apr 4;16:74. doi: 10.1186/s12943-017-0637-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PinX1 suppresses cell proliferation and clonogenicity in NSCLC cell lines. a Western blotting analysis showed that the endogenous levels of PinX1 in four NSCLC cell lines (SK-MES-1, H125, A549, H1299) were lower than that in control normal lung tissues. NT, normal lung tissues. b PinX1 siRNA was introduced into two NSCLC cell lines, H1299 and A549. pCDH-PinX1 lentiviral were transuded into SK-MES-1. PinX1, Bcl-2, and Bax protein levels were assessed by using western blot analysis. c MTT assay was performed to measure viability of cells expressing different levels of PinX1. d Colony-forming assay was used to observe colony forming ability of cells expressing different levels of PinX1. Each bar represents the mean ± SD of three independent experiments. *, compared to control group (P < 0.05)