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. 2017 Apr 4;16:74. doi: 10.1186/s12943-017-0637-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PinX1 arrestd G1/S phase transition of the cell cycle. a EdU incorporation assay was used to analyze the population of S phase cells expressing different levels of PinX1. Nuclei were stained with DAPI (blue). S phase cells were stained with EdU (red). Merged images show the percentage of S phase in NSCLC cells. b EdU positive cells were represented using scattergram in NSCLC cells expressing different levels of PinX1. At least 300 total cells from triplicate experiments were counted for each group. *, compared to corresponding control group (P < 0.05). c Cell cycle phases in NSCLC cells expressing different levels of PinX1 were determined by flow cytometry assay. d G1/G0, S, and G2/M phase were plotted in NSCLC cells using histogram. *, compared to corresponding control group (P < 0.05)