Figure 8. Immunolabeling of dDAVP induces the translocation of Claudin 4 into the TJs of the ES epithelia cells.

Confocal LSM analysis of the subcellular translocation of Claudin 4 in cryosections of the rat p4 ES epithelium. (a–a”’) After treatment with the V2R antagonist H9400 Claudin 4 labeling was observed in the cytoplasm of ES epithelial cells. (b–b”’) After dDAVP treatment, intracellular labeling was markedly reduced, whereas co-localization with ZO-1 in the TJs and labeling of the basolateral membranes has increased. Scale bar 5 μm.