Table 1. Assesment of absolute Claudin mRNA expression in rat p4 ED, ES and ST.

Gene	ED	ES	ST	Gene	ED	ES	ST	Gene	ED	ES	ST
Cldn1	+	++	+	Cldn9	+	+	(+)	Cldn18	n.d.	n.d.	n.d.
Cldn3	(+)	(+)	(+)	Cldn10	++++	+++++	++	Cldn19	(+)	(+)	++
Cldn3	+++	++++	+	Cldn11	++++	+++	++++	Cldn20	++	++	++
Cldn4	+++	++++	+	Cldn12	+	++	+	Cldn22	+	+	++
Cldn5	+	+	++	Cldn14	n.d.	n.d.	n.d.	Cldn23	+	+	+
Cldn6	+++	++++	+	Cldn15	+	+	+	Cldn24	+	+	+
Cldn7	++	+++	+	Cldn16	++	+++	+	Pend	+++	+++++	++
Cldn8	+++++	+++++	++	Cldn17	++	++	(+)	UBC	++++	++++	++++

Ct ≤ 11: +++++ very high expression; 14 < Ct ≤ 17: +++ moderate expression; 17 < Ct ≤ 20: ++ low expression; 20 < Ct ≤ 24: ++ very low expression; Ct > 24: (+) extremely low expression; n.d.: not detected.