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. 2017 Apr 4;7:45936. doi: 10.1038/srep45936

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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One C-terminus truncated ORF and three intact ORFs are included in a 4.4-kb DNA fragment inserted into the recombinant plasmid pUC-DP61, each of which is preceded by a respective promoter-like sequence and a respective Shine-Dalgarno (SD) sequence. A predicted promoter sequence (−35 region and −10 region) and Shine-Dalgarno (SD) sequence, the initiation codon TTG of ORF3 designated UPF0118 (Genbank accession No. KY231907), the enzymatic site of SalI and EcoRI are underlined. The stop codon TAA of ORF3 is indicated by the asterisk and a possible terminator following ORF3 indicated by inverted solid arrows. UPF0118 was subcloned, together with its promoter-like and SD sequences, into SalI- and EcoRI-digested pUC18 through digestion by the same two restriction enzymes and ligation.