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. 2017 Apr 4;7:45936. doi: 10.1038/srep45936

Figure 4. Salt tolerance and alkaline pH resistance of E. coli strains KNabc.

Figure 4

For the salt tolerance test, 1% overnight cultures of KNabc transformant cells carrying the empty vector pUC18 (white column), pET-22b (lightgrey column), pUC-UPF0118 (darkgrey column) or pET-22b-UPF0118 (black column) grown at 37 °C in the LBK medium at pH 7.0 were innoculated into fresh LBK medium at pH 7.0, to which NaCl (A) or LiCl (B) was added at indicated concentrations, followed by incubation at 37 °C. To test the effect of pH (C) on cell growth, 1% overnight cultures of the above mentioned KNabc transformant cells grown at 37 °C in the LBK medium at pH 7.0 were innoculated into fresh LBK medium containing 50 mM NaCl at indicated pHs adjusted by adding the Hepes-Tris buffer at the final concentration of 100 mM, followed by incubation at 37 °C. The above mentioned cell growth was ended after 24 h and monitored turbidimetrically at 600 nm. Each data point represents the average of three independent determinations.