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Experimental protocol. The InsG100 group received 0.5 U/L insulin, 100 mg/dL glucose and 27.5 mmol/L of mannitol in KH buffer. The InsG600 group received 0.5 U/L insulin and 600 mg/dL glucose in KH buffer. The G600 group was perfused with KH buffer containing 600 mg/dL
