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. 2017 Apr 4;23:22. doi: 10.1186/s40409-017-0112-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig 6 — Neutralization of hemorrhagic effects of D. acutus venom by IgY (mean ± SD, n = 4). Mixtures containing a constant amount of venom and various dilutions of IgY were incubated at 37 °C for 30 min. Afterwards, aliquots of the mixtures (10 μL containing 3 MHD of venom) were injected subcutaneously into the dorsal skin of mice, whereas control group received the same amount of venom with normal yolk. Hemorrhage was expressed as a percentage, taking as 100% the diameter of the lesions induced by inoculating 10 MHD of venom alone