Table 1.
Gene | Median Cta | Median ∆Ctb | Slopec | (R2)d | Efficiencye |
---|---|---|---|---|---|
CCL2 | 28.62 | 9.66 | −3.29 | 0.997 | 2.011 |
CCL5 | 28.95 | 10.05 | −3.28 | 0.991 | 2.019 |
CXCL6 | 24.29 | 5.23 | −3.19 | 0.999 | 2.060 |
CXCL8 | 29.26 | 10.34 | −3.11 | 0.994 | 2.097 |
IFIT3 | 24.96 | 6.04 | −3.07 | 0.993 | 2.117 |
IL6 | 29.11 | 10.12 | −3.34 | 0.993 | 1.992 |
IRF3 | 24.16 | 5.27 | −3.09 | 0.991 | 2.108 |
MYD88 | 24.62 | 5.71 | −3.02 | 0.991 | 2.143 |
NFKB1 | 26.58 | 7.62 | −2.91 | 0.996 | 2.204 |
PTGS2 | 27.47 | 8.49 | −3.06 | 0.986 | 2.120 |
TLR2 | 28.51 | 9.64 | −3.31 | 0.999 | 2.006 |
TLR4 | 30.20 | 11.28 | −2.94 | 0.999 | 2.189 |
TOLLIP | 23.59 | 4.75 | −3.35 | 0.995 | 1.989 |
aThe median is calculated considering all time points and treatments
bThe median of ∆Ct is calculated as [Ct gene - geometrical mean of Ct internal controls] for each time point and treatment
cSlope of the standard curve
dR2 stands for the coefficient of determination of the standard curve
eEfficiency is calculated as [10(−1/Slope)]