Table 2.

Log₂ back-transformed LSM of gene transcription for treatment (TRT) and incubation time (T), SEM and P values for TRT and T

	LSM TRTd				LSM T		SEM		P-value
Gene	Control	LPS	LTA	L + L	3 h	6 h	TRT	T	TRT	T
Cytokines
CCL2	0.49c	1.61a	0.54c	0.80b	0.72	0.80	0.08	0.06	<0.0001	0.0637
CCL5	1.64b	1.58b	1.65b	1.91a	1.60z	1.78y	0.06	0.04	0.0022	0.0034
CXCL6	0.43c	1.58a	0.40c	0.96b	0.78y	0.66z	0.08	0.06	<0.0001	0.0093
CXCL8	0.51c	1.64a	0.43c	0.97b	0.97y	0.61z	0.12	0.09	<0.0001	<0.0001
IL6	1.26b	2.65a	1.27b	1.90a	1.66	1.71	0.22	0.18	0.0004	0.8208
Regulatory genes
IFIT3	1.01c	1.13b	1.20ab	1.24a	0.95z	1.37y	0.04	0.03	<0.0001	<0.0001
IRF3	1.05b	1.09	1.13	1.19a	1.01z	1.22y	0.05	0.04	0.0818	<0.0001
MYD88	1.73b	2.05a	1.81b	2.11a	1.81z	2.03y	0.03	0.03	<0.0001	<0.0001
NFKB1	1.13c	1.49a	1.34b	1.51a	1.10z	1.68y	0.05	0.04	<0.0001	<0.0001
PTGS2	1.05b	1.30a	1.32a	1.29a	1.03z	1.48y	0.05	0.04	<0.0001	<0.0001
TLR2	10.39	11.38	10.11	11.24	10.58	10.96	0.09	0.07	0.3747	0.5420
TLR4	1.04c	1.49a	1.04c	1.22b	1.18	1.19	0.08	0.06	<0.0001	0.8645
TOLLIP	0.96b	0.96b	1.04a	0.95b	0.98	0.97	0.02	0.01	<0.0001	0.3915

^a-cDifferent letters represent significant differences between treatments (P < 0.05)

The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c

^dTreatments: Control = incubation without toxins; LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins

^y-zDifferent letters represent significant differences between time points (P < 0.05). The letter y indicates higher transcript levels than z