Table 3.

Log₂ back-transformed LSM of interactions between treatment (TRT) and incubation time (T) on gene transcription, SEM and P values for TRT × T

		LSM TRTd × T				SEM	P value
Gene	T	Control	LPS	LTA	L + L	TRT × T	TRT × T
Cytokines
CCL2	3 h	0.45c	1.83a,y	0.48c,z	0.69b,z	0.11	0.0040
	6 h	0.53c	1.41a,z	0.61c,y	0.94b,y
CCL5	3 h	1.40c,z	1.45bc,z	1.62b	2.00a	0.07	0.0018
	6 h	1.91y	1.72y	1.68	1.83
CXCL6	3 h	0.43c	1.67a	0.51c,y	0.99b	0.12	0.0274
	6 h	0.44c	1.49a	0.32d,z	0.93b
CXCL8	3 h	0.49c	2.17a,y	0.62c,y	1.34b,y	0.17	0.0085
	6 h	0.52b	1.24a,z	0.30c,z	0.70b,z
IL6	3 h	1.01b	3.01a	1.15b	2.17a	0.28	0.1423
	6 h	1.57	2.33	1.41	1.66
Regulatory genes
IFIT3	3 h	0.85b,z	0.96a,z	0.96a,z	1.05a,z	0.05	0.5151
	6 h	1.19b,y	1.34a,y	1.48a,y	1.47a,y
IRF3	3 h	0.94b,z	1.01z	0.99z	1.12a	0.07	0.3942
	6 h	1.18y	1.18y	1.28y	1.26
MYD88	3 h	1.64b,z	1.95a,z	1.67b,z	2.02a	0.05	0.7335
	6 h	1.82b,y	2.16a,y	1.96b,y	2.20a
NFKB1	3 h	0.93b,z	1.18a,z	1.12a,z	1.21a,z	0.06	0.5318
	6 h	1.37c,y	1.88a,y	1.62b,y	1.90a,y
PTGS2	3 h	0.85b,z	1.12a,z	1.07a,z	1.12a,z	0.07	0.2535
	6 h	1.31b,y	1.50y	1.63a,y	1.48y
TLR2	3 h	9.46	10.76	10.80	11.41	0.12	0.2028
	6 h	11.41	12.04	9.47	11.07
TLR4	3 h	0.99c	1.42a	1.28ab,y	1.08bc,z	0.10	<0.0001
	6 h	1.09b	1.57a	0.85c,z	1.37a,y
TOLLIP	3 h	0.96b	0.97b	1.03a	0.97b	0.03	0.3689
	6 h	0.96b	0.94b	1.06a	0.94b

^a-cDifferent letters represent significant differences between treatments within the same incubation time (P < 0.05). The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c

^dTreatments: LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins; Control = incubation without toxins

^y-zDifferent letters represent significant differences between time points within the same treatment (P < 0.05). The letter y indicates higher transcript levels than z