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. 2017 Apr 4;17:38. doi: 10.1186/s12886-017-0433-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — A photomicrograph of a section of a rat’s retina. a Control negative group shows localized positive GFAP immunohistochemical staining confined to GCL, NFL, and ILM. b, c Control positive group (I) and (II) respectively show positive GFAP staining of Muller cells radial processes extended throughout the whole retinal thickness; from the ILM to the OLM (black arrows). d Eye received intravitreal injection of 0.02 ml of 2-Methoxyestradiol (10 μg/ ml) and (e) Eye received intravitreal injection of 0.02 ml of 2-Methoxyestradiol (100 μg/ml) showing limited GFAP expression confined only to GCL, NFL, and ILM (black arrows on) d (original magnification X400)