Fig. 3.

Cell Objects extraction and classification. a Each colony is an ensemble of cell objects corresponding to one or more “touching” cells; e.g. see three cell objects marked with color. We first extract the skeleton of each object and classify it as complex (red) or collinear (green) according to the presence (or absence) of skeleton junctions (i.e. skeleton pixels with more than two neighbors). b The skeleton of the smaller red object has two junctions (marked with yellow boxes) so it is classified as a complex object. c Colony object with no junctions (green) classified as collinear object. d A complex object (large red) with four junctions. See text for details