Fig. 2.

Gene-specific cMNR frameshift mutations in exosomal and cellular DNA of different MSI CRC cell lines. Frameshift mutations are recognized as shifts in allele length as determined by DNA fragment analysis. In the reference cMNR peak pattern of the microsatellite stable cell line SW948 (control) the highest peak refers to the normal wildtype (wt) allele length (red vertical line; reference mark), whereas additional peaks represent PCR-associated artefact peaks. Allele length shifts (-1, -2) were scored if novel peaks were obtained in cell lines compared to the microsatellite stable control SW948 cell line, or if the ratio of peak areas of corresponding peaks in cell lines and normal control revealed values ≤0.5 or ≥2. Differences between exosomal and cellular mutant allele pattern (circled) are indicated. Allele sizes are given by numbers