Table 1. X-ray diffraction data-collection and processing statistics.
Values in parentheses are for the highest resolution shell.
| Aae Hfq, apo form (‘P1’) | Aae Hfq·U6 RNA (‘P6’) | |
|---|---|---|
| Diffraction source | 24-ID-E, APS NE-CAT | 24-ID-C, APS NE-CAT |
| Wavelength (Å) | 0.9792 | 0.9195 |
| Temperature (K) | 100 | 100 |
| Detector | ADSC Q315 CCD | Dectris PILATUS 6MF |
| Crystal-to-detector distance (mm) | 200 | 300 |
| Rotation range per image (°) | 1.0 | 1.0 |
| Total rotation range (°) | 400.0 | 300.0 |
| Exposure time per image (s) | 1.0 | 1.0 |
| Space group | P1 | P6 |
| a, b, c (Å) | 63.46, 66.06, 66.10 | 66.19, 66.19, 34.21 |
| α, β, γ (°) | 60.05, 83.94, 77.17 | |
| Mosaicity (°) | 0.143 | 0.107 |
| Resolution range (Å) | 57.27–1.49 (1.53–1.49) | 34.21–1.50 (1.55–1.50) |
| Total No. of reflections | 299450 | 46203 |
| No. of unique reflections | 138120 | 13177 |
| Completeness (%) | 93.7 (83.7) | 94.9 (93.4) |
| Multiplicity | 2.2 (2.1) | 3.5 (3.5) |
| 〈I/σ(I)〉 | 14.0 (3.4) | 12.3 (3.6) |
| R merge † | 0.039 (0.258) | 0.056 (0.292) |
| R meas ‡ | 0.052 (0.349) | 0.065 (0.345) |
| R p.i.m. ‡ | 0.035 (0.234) | 0.032 (0.179) |
| CC1/2 § | 0.998 (0.886) | 0.998 (0.942) |
| Overall B value from Wilson plot (Å2) | 12.62 | 15.87 |
| Matthews coefficient V M (Å3 Da–1) | 2.06 [12 subunits in asymmetric unit] | 2.28 [one subunit in asymmetric unit] |
| Solvent content (%) | 40.21 | 46.08 |
†
R
merge = 
, where Ii(hkl) is the intensity of the ith observation of reflection hkl, 〈.〉 denotes the mean of symmetry-related (or Friedel-related) reflections and the coefficient α = 1; the outer summations run over only unique hkl with multiplicities greater than one.
‡
R meas is defined analogously to R merge, save that the prefactor α = [Nhkl/(Nhkl − 1)]1/2 is used; Nhkl is the number of observations of reflection hkl (index i = 1→Nhkl). Similarly, the precision-indicating merging R factor, R p.i.m., is defined as above but with the prefactor α = [1/(Nhkl − 1)]1/2.
§
CC1/2 is the correlation coefficient between intensities chosen from random halves of the full data set.