Table 1. Primer sequences.
| Species | Gene | Forward | Reverse |
|---|---|---|---|
| Human | FLI1 | GGATGGCAAGGAACTGTGTAA | GGTTGTATAGGCCAGCAG |
| AIRE | GAGAGTGCTGAGAAGGACA | GTTTAATTTCCAGGCACATGA | |
| IL1A | ATCAGTACCTCACGGCTGCT | TGGGTATCTCAGGCATCTCC | |
| K6 | CTGAATGGCGAAGGCGTT | CCACTGCCGACACCACT | |
| K16 | GACCGGCGGAGATGTGAAC | CTGCTCGTACTGGTCACGC | |
| CTGF | TTGCGAAGCTGACCTGGAAGAGAA | AGCTCGGTATGTCTTCATGCTGGT | |
| SNAI1 | ACCCCAATCGGAAGCCTAACT | GGTCGTAGGGCTGCTGGAA | |
| CDH1 | ACAACAAGCCCGAATTCACCCA | TCACAGCTGTTGCTGTTGTGCT | |
| GAPDH | ACCCACTCCTCCACCTTTGA | CATACCAGGAAATGAGCTTGACAA | |
| Mouse | Il1a | ATGATCTGGAAGAGACCATCC | GGCAACTCCTTCAGCAACA |
| Il1b | TTGACGGACCCCAAAAGAT | GAAGCTGGATGCTCTCATCTG | |
| Il6 | GATGGATGCTACCAAACTGGAT | CCAGGTAGCTATGGTACTCCAGA | |
| Il8 | CTGGCCGTGGCTCTCTTG | CCTTGGCAAAACTGCACCTT | |
| Il13 | GGATATTGCATGGCCTCTGTAAC | AACAGTTGCTTTGTGTAGCTGA | |
| Il17a | CTCCAGAAGGCCCTCAGACTAC | AGCTTTCCCTCCGCATTGACACAG | |
| Tnfa | ACCCTCACACTCAGATCATCTTC | TGGTGGTTTGCTACGACGT | |
| Ccl2 | CATCCACGTGTTGGCTCA | GATCATCTTGCTGGTGAATGAGT | |
| Ifng | TCAAGTGGCATAGATGTGGAAGAA | TGGCTCTGCAGGATTTTCATG | |
| Ctgf | GTGCCAGAACGCACACTG | CCCCGGTTACACTCCAAA | |
| Tgfb1 | GCAACATGTGGAACTCTACCAGAA | GACGTCAAAAGACAGCCACTCA | |
| Col1a1 | GCCAAGAAGACATCCCTGAAG | TGTGGCAGATACAGATCAAGC | |
| Col1a2 | GGAGGGAACGGTCCACGAT | GAGTCCGCGTATCCACAA | |
| Adamts2 | AGTGGGCCCTGAAGAAGTG | CAGAAGGCTCGGTGTACCAT | |
| Lum | AGATGCTTGATCTTGGAGTAAGA | CAATGAACTTGAAAAGTTTGATG | |
| Dcn | TGAGCTTCAACAGCATCACC | AAGTCATTTTGCCCAACTGC | |
| K6 | TTCTCTACTTCCCAGCCTTCTCA | GCCACGGTGGCTGGTTT | |
| K16 | GACCTCAGCCGCATTCTGA | GCGAAGCTGAGCCAGCTGCT | |
| Snai1 | CAACTATAGCGAGCTGCAGG | ACTTGGGGTACCAGGAGAGAGT | |
| Ins2 | AGACCATCAGCAAGCAGGTC | CTGGTGCAGCACTGATCCAC | |
| Csna | TGACTGGACCCTCCATTCTC | CCTTGATTCTCTCCGCTCAG | |
| Chrna1 | GTGCTGGGCTCTTTCATCTC | TTCTGTGCGCGTTCTCATAC | |
| s100a8 | GGAAATCACCATGCCCTCTA | GCTGTCTTTGTGAGATGCCA | |
| Tg | CTGTGGTGTTCTCAGCCTCAACTCC | TTGGCCTGAGTAGCAGAGGT | |
| Spt1 | TTGTGTTGCTTGGTGTTT | TCGACTGAATCAGAGGAATCAACT | |
| Gad67 | CACAAACTCAGCGGCATAGA | CTGGAAGAGGTAGCCTGCAC | |
| Col2a1 | ACTGGTAAGTGGGGCAAGAC | CCACACCAAATTCCTGTTCA | |
| K10 | AACAGATTCGGGCTGAGACC | TTCTAGCAGGCTGCGGTAGG | |
| Crp | TACTCTGGTGCCTTCTGATCATGA | GGCTTCTTTGACTCTGCTTCCA | |
| Aire | GTACAGCCGCCTGCATAGC | CCCTTTCCGGGACTGGTTTA | |
| Fli1 | ACTTGGCCAAATGGACGGGACTAT | CCCGTAGTCAGGACTCCCG | |
| Gapdh | CGTGTTCCTACCCCCAATGT | TGTCATCATACTTGGCAGGTTTCT | |
| SNAI1 ChIP primer | Amplified Region | ||
| –715/–472 | AGAAGCTACCCTTCGGGAGA | GCATTGACGAGGGAAACG | |
| AIRE ChIP primer | Amplified Region | ||
| 1 | –403/–235 | CAGGACAGGGCCACATTC | AGGGACACTTCCCTGTGATG |
| 2 | –587/–389 | TAGGGGCTCTCAGCTTGTGT | GAATGTGGCCCTGTCCTG |
| 3 | –807/–634 | CTTTGCTCTTTGCGTGGTC | CCTCAGAAGCCGGCGTAG |
| 4 | –1038/–759 | CACTGTCCCCACTCACGTC | CTTTAAAAGGGGCACACTCG |
| 5 | –1229/–1036 | GACCTCTCGTGGTTCTCTGC | TGCCCCCAGTAAAACAAGAC |
The sequences of the primers used for qRT-PCR and ChIP of SNAI1 and AIRE gene promoter regions are shown. The nucleotide sequences of the primers are listed in the 5′ to 3′ direction.