Figure 6.

Decreased vaginal inflammatory monocyte infiltration in Ifnar^−/− mice during HSV-2 infection. (A) WT, Ifnar^−/−, and Il18^−/− mice were infected with 10⁴ pfu HSV-2, and vaginal tissue was collected at baseline and on days 0–3 p.i. and examined for inflammatory monocytes, defined as CD45⁺CD11c⁻CD11b⁺Ly6G⁻Ly6C^hiCCR2⁺ cells. Day 2 and 3 p.i. data are shown in representative flow plots. (B) Flow cytometry data were quantified and graphically represented (n = 3; repeated once with similar results). (C) Vaginal lavages were collected on days 0–3 p.i. and examined for MCP-1 (n = 3; repeated once with similar results). (D) WT and Il18^−/− mice were infected with HSV-2 ivag, and vaginal tissue was collected at baseline and on days 2 and 3 p.i. and examined for inflammatory monocytes (n = 3). (E) WT and Il18^−/− mice were infected with 10⁴ pfu HSV-2 ivag, and on days 0–3 p.i., vaginal lavages were examined for MCP-1 (n = 4). Data in B–E are displayed as mean ± SEM and were analyzed using two-way ANOVA: **, P < 0.01; ***, P < 0.001.