Figure 7.

Inflammatory monocytes are required for activating NK cell IFN-γ production during HSV-2 infection. (A and B) WT B6 mice were given an anti–GR-1 antibody or its respective isotype-matched control Ig and infected with 10⁴ pfu HSV-2 ivag. Vaginal tissue was examined for neutrophil (CD45⁺CD11c⁻CD11b⁺Ly6G⁺) and inflammatory monocyte (CD45⁺CD11c⁻CD11b⁺Ly6G⁻Ly6C^hiCCR2⁺) depletion on day 3 p.i. Representative flow plots of vaginal neutrophil and inflammatory monocyte populations are shown (respectively in A and B). (C and D) Vaginal neutrophil (C) and inflammatory monocyte (D) populations are displayed graphically (n = 5; repeated once with similar results). (E) On days 1–3 p.i., vaginal washes were examined for IFN-γ (n = 5; repeated twice with similar results). (F and G) Vaginal tissue collected on day 3 p.i. was also examined for total vaginal NK cell number (F; CD45⁺CD3⁻NK1.1⁺; n = 5) and proportion of CD11c⁺ cells (G; CD45⁺CD11c⁺; n = 5; repeated once with similar results). Data in C, D, F, and G are displayed as mean ± SEM and were analyzed using an unpaired Student’s t test in D and G and a Mann–Whitney test (for nonparametric data) in C and F: n.s., not significant; **, P < 0.01; ***, P < 0.001. Data in E are displayed as mean ± SEM and were analyzed using two-way ANOVA: ****, P < 0.0001.