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. 2017 Apr 3;214(4):973–989. doi: 10.1084/jem.20161015

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© 2017 Hu et al.

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Figure 3. — TRIM38 catalyzes sumoylation of RIG-I and MDA5. (A) Effects of TRIM38 on polyubiquitination of RIG-I, MDA5, and TRIF. HEK293 cells were transfected with the indicated plasmids for 24 h, followed by immunoprecipitation and immunoblotting analysis with the indicated antibodies. (B and C) Effects of TRIM38 on sumoylation of RIG-I, MDA5, and TRIF. HEK293 cells were transfected with the indicated plasmids for 24 h, followed by Ni²⁺ pull-down assays and immunoblotting analysis. (D) Effects of Trim38 deficiency on sumoylation of endogenous RIG-I. Trim38^+/+ and Trim38^−/− MLFs were left uninfected or infected with SeV for the indicated times, followed by immunoprecipitation and immunoblotting analysis. *, sumoylation bands of RIG-I. (E) Effects of Trim38 deficiency on sumoylation of endogenous MDA5. Trim38^+/+ and Trim38^−/− MLFs were left uninfected or infected with EMCV for the indicated times, followed by immunoprecipitation and immunoblotting analysis. *, sumoylation bands of MDA5. Data are from one representative experiment. All experiments were repeated three times.