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. 2017 Apr 3;214(4):1181–1198. doi: 10.1084/jem.20161263

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© 2017 Inoue et al.

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Figure 3. — Assessment of Foxo1 requirement for GC maintenance and proliferation in single transfer experiments. (A) Schematic illustration of the experimental protocol. (B) Flow cytometry of NP-specific donor B cells (CD45.1⁺B220⁺NP⁺). (C) Histograms representing the number of donor IgG1⁻ GC B cells (CD45.1⁺B220⁺NP⁺CD38⁻IgG1⁻) and IgG1⁺ GC B cells (CD45.1⁺B220⁺NP⁺CD38⁻IgG1⁺) in 10⁶ splenocytes (left), and the ratio of DZ:LZ cells (right). n = 5 biological replicates. (D) Proliferation status of Foxo1^+/+ and Foxo1^f/f LZ GC B cells assessed by EdU incorporation 30 min after an EdU injection. n = 3 biological replicates. (E) Analysis of Batf mRNA expression in Foxo1^+/+ DZ, LZ, and Foxo1^f/f GC B cells by real-time qPCR. n = 3 biological replicates. Error bars represent SD. Data are representative of three (B and C) or two independent experiments (D and E). **, P < 0.01; ***, P < 0.001; unpaired Student’s t test.