Figure 4.

GC phenotype with Foxo1 ablation during a polyclonal immune response. (A) Schematic representation of the experimental protocol. Because entering the GC in the polyclonal stimulation was a little earlier than the monoclonal condition (Fig. 2 A), we treated mice with tamoxifen on day 6, 7, and 8, and analyzed on day 11. (B, top) Flow cytometry of donor B cells (B220⁺CD45.1⁻CD45.2⁺ and B220⁺CD45.1⁺CD45.2⁻). (bottom left) Percentage of each donor in total donor GC B cells (CD45.1⁻CD45.2⁺ or CD45.1⁺CD45.2⁻B220⁺GL7⁺CD38⁻). (bottom right) Ratio of DZ:LZ cells. n = 5 biological replicates. Error bars represent SD. *, P < 0.05; ***, P < 0.001; paired Student’s t test. Data are representative of two independent experiments.