Figure 3.

Eros^−/− neutrophils and macrophages have a severely impaired phagocyte respiratory burst in vitro. (A–C) ROS production (three technical replicates, each control and Eros^−/− sample is pooled from two mice) measured in relative light units (RLU) by purified BM neutrophils from control or Eros^−/− mice in response to (A) fMLP, (B) PMA, or (C) zymosan. Data are representative of at least three independent experiments. (D and E) ROS production (replicates from BM derived from three individual mice, measured in RLU) by purified BM-derived macrophages from control or Eros^−/− mice in response to PMA (D) and zymosan (E). (F) ROS production by peritoneal cells from Rag^−/− (three mice) or Eros^−/− Rag^−/− mice (three mice) in response to PMA. Data are representative of at least three independent experiments. Area under the curve (AUC) was calculated for each sample. Error bars represent the SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, analyzed by unpaired Student’s t test.