Table 1. DR-associated endothelial ligands identified by comparative ligandomics.
CCDS ID | Protein | Binding activity | Activity Ratio | |
---|---|---|---|---|
DR | Control | |||
DR-high ligands with increased binding to diabetic ECs | ||||
CCDS23347.1 | Scg3a | 1,731 | 0 | 1,732 |
CCDS18810 | C1qba | 837 | 0 | 838 |
CCDS15031 | Fn1a | 419 | 0 | 420 |
CCDS35631 | Col4a3a | 409 | 2 | 137 |
CCDS28285 | APPa | 206 | 1 | 104 |
CCDS22638 | Cdh1a | 132 | 0 | 133 |
DR-low ligands with decreased binding to diabetic ECs | ||||
CCDS40011 | HRP-3a | 48 | 11,140 | 0.0044 |
CCDS17457 | HDGFa | 0 | 83 | 0.0119 |
Internal positive and negative controls | ||||
VEGF-Phage | 408 | 2,420 | 0.1689 | |
GFP-Phage | 10 | 10 | 1 | |
Total identified sequences | 489,126 | 473,965 | ||
Total identified ligands | 1,548 | 844 | ||
Diabetes-related ligandsa | 353↑ | 105↓ |
P < 0.001; DR versus control; χ2 test. Activity ratio = (DR + 1)/(Control + 1).