Table 1. Generation of transgenic founder rats.

Summary of injection
	Number of
	Injected	Transferred	Born		Tg(+) rats
Gene	eggs	eggs	offspring
pMGTsc2-GSM (G1556S-type)	135	127	53	5|	4(Tsc2+/+)
					1(Tsc2+/−)
pMGTsc2-NSM (N525S-type)	236	214	73	0
pMGTsc2-WT (Wild-type)	172	106	21	5|	1(Tsc2+/+)
					4(Tsc2+/−)

*Donor animals: Eker(Tsc2+/−) ♂ x Wistar(Tsc2+/+).

^†Phenotype of rats carrying GSM- or WT-Tg were previously reported²³.