Figure 2. Comparing outcomes from manual versus automatic cell detection methods.

Comparative outcomes of modulating functional IMPα2 levels on PSPC1 nuclear transport and paraspeckle localization in HeLa cells. Cells were transiently transfected with constructs encoding GFP-tagged IMPα2 variants as indicated (see Fig. 1A for predicted function). Paraspeckles were identified using indirect immunofluorescence to detect endogenous PSPC1. These measures were assessed within groups for the entire cell population (A), per foci positive cell populations (B) or on a per foci basis (C). Most measures are shown as geometric means (GM); error bars represent 95% confidence intervals.