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. Author manuscript; available in PMC: 2017 Apr 4.

Published in final edited form as: J Phys Chem B. 2016 Sep 12;120(37):9922–9934. doi: 10.1021/acs.jpcb.6b07248

Table 2.

DXS Steady-State Kinetics Data (Wild-Type and Mutants) for Both Pyruvate and G3P^a

		Pyruvate
	K_M (mM)	k_cat/K_M (s⁻¹ M⁻¹)	k_cat (s⁻¹)	% WT
wild-type	0.28 ± 0.03	2.6 × 10⁴	7.4 ± 0.3	100
H82A	0.23 ± 0.02	1.7 × 10³	0.38 ± 0.01	5.1
H304A	1.7 ± 0.5	5.8 × 10²	0.90 ± 0.01	12.1
D430A	0.52 ± 0.5	1.4 × 10⁴	7.2 ± 0.2	97.3
H434A	1.7 ± 0.1	5.9 × 10³	9.9 ± 0.2	133.8

		G3P
	K_M (mM)	k_cat/K_M (s⁻¹ M⁻¹)	k_cat (s⁻¹)	% WT

wild-type	0.05 ± 0.01	1.5 × 10⁵	7.9 ± 0.4	100
H82A	0.03 ± 0.01	1.3 × 10⁴	0.37 ± 0.02	4.7
H304A	0.08 ± 0.02	1.1 × 10⁴	0.90 ± 0.1	11.4
D430A	0.12 ± 0.01	6.6 × 10⁴	7.7 ± 0.2	97.5
H434A	0.23 ± 0.01	4.2 × 10⁴	9.6 ± 0.3	121.5

^a

% WT was determined by comparing the mutant k_cat to the wild-type k_cat.