D-OH microglia localization was accompanied with microglial activation. Low magnification images (top) showed that at G29, most D-OH distributed around the ventricle region, while at PND1, D-OH specifically accumulated at the ventricle horn (pointed out by the arrow). Magnification of the ventricle horn (bottom) showed that at G29, a hint of microglial localization was observed, while at PND1, D-OH was selectively localized within the activated microglial cells. Red: D-OH; green: anti-Iba1 labeled microglial cells; blue: DAPI. Scale bar: 400 μm.