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. Author manuscript; available in PMC: 2017 Apr 4.
Published in final edited form as: Nat Protoc. 2015 Oct 22;10(11):1802–1819. doi: 10.1038/nprot.2015.119

Figure 2.

Figure 2

Mechanical tissue stabilizers. Mechanical tissue stabilizers can guarantee reproducibility in the motion over a physiological cycle during intravital microscopy imaging. (a) 3D-printed stabilizer used in the current protocol. The presence of a grid bonded to the pericardium provides efficient suppression of motion artifacts for identification and tracking of individual cardiomyocytes. (b) Suction-based stabilizer. Copyright 2012, from ‘Motion compensation using a suctioning stabilizer for intravital microscopy’ (ref. 15) by Vinegoni, C. et al. Adapted by permission of Taylor & Francis LLC, http://www.tandfonline.com.