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. Author manuscript; available in PMC: 2017 Apr 4.
Published in final edited form as: Nat Protoc. 2015 Oct 22;10(11):1802–1819. doi: 10.1038/nprot.2015.119

Figure 7.

Figure 7

Schematic of the cardiac intravital microscopy system for confocal and two-photon imaging. Timing waveforms from an animal ventilator and an ECG monitoring amplifier are recorded in combination with the ‘Frame Active’ signal from the microscopy PSU unit. When you are performing prospective sequential segmented microscopy, a triggered pacemaker device is also inserted (red wirings in the figure) to actively pace the heart at a frequency slightly different from the microscope acquisition frequency.