TABLE 1.

Fluorescent reporter options.

Structure or physiology	Fluorescent reporter	Dosing and instructions (see Reagents for supplier information)
Capillary networks	Lectin: fluorescein or rhodamine label	Inject 50 μl of the 2 mg/ml stock solution directly by tail vein and wait for 10–15 min before imaging. Excite fluorescein label with a 488-nm laser and rhodamine with a 559-nm laser
Blood flow	Angiosense-680-IVM	Inject 150 μl at a dye concentration of 2 mol per mouse via the tail vein. Imaging can be performed within minutes of injection. Excite fluorescence using a 635-nm laser
Cardiomyocytes	Autofluorescence Cell membrane stain di-2-anepeq	Excite endogenous autofluorescence with a 488-nm laser. Inject 40 μl of the 5 mM stock solution directly via the tail vein and wait for 15 min before imaging. Excite one-photon fluorescence using a 488-nm laser or two-photon fluorescence using a 910-nm center wavelength on the Ti:Sapphire pulsed laser
Cell nuclei	Hoechst 33258	Apply 50 μl of a 1/10,000 solution of Hoechst 33258 topically to the myocardial surface. Excite fluorescence using a 405-nm laser
Circulating leukocytes	Rhodamine-6G	Inject 0.2 mg/kg intravenous by tail vein and wait for 10–15 min before imaging. Excite fluorescence using a 559-nm laser
Tissue macrophages	CLIO-VT680	Inject 15 mg of Fe per kg via the tail vein 12–24 h before imaging. Excite fluorescence using the 635-nm laser