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DLD1 cells were treated with control media or media containing 3.3 μM 5C6 for 1.5 hours. Cells were then washed, fixed, and immunostained for presence of IgG, followed by counterstaining with PI to allow visualization of the nucleus. Light and fluorescent images under GFP and RFP filters are presented. Merged images confirm nuclear localization by 5C6. Scale bar: 20 µm.
