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. 2014 Aug 6;4:5967. doi: 10.1038/srep05967

Figure 5. α2AP induced the production of TGF-β.

Figure 5

(A) The renal fibroblasts were stimulated by α2AP (1, 2, 4 nM) for 24 hours. The expression of type I collagen, fibronectin (FN), TGF-β was measured by a Western blot analysis. The blots were cropped, and the full-length blots are presented in the supplementary information. The histogram on the right panels shows quantitative representations of type I collagen, FN, and TGF-β expression obtained from densitometry analysis (n = 3). (B) The renal fibroblasts were stimulated by α2AP (1, 2, 4 nM) for 24 hours. Phosphorylation of smad2/3 was measured by a Western blot analysis. The blots were cropped, and the full-length blots are presented in the supplementary information. The histogram on the right panels shows quantitative representations of phospho-smad2/3 expression obtained from densitometry analysis (n = 3). (C) The renal fibroblasts were stimulated with 2 nM α2AP for the indicated periods. The expression of TGF-β was measured by a Western blot analysis. The blots were cropped, and the full-length blots are presented in the supplementary information. The histogram on the right panels shows quantitative representations of TGF-β expression obtained from densitometry analysis (n = 3). (D) The renal fibroblasts were pretreated with DMSO or 30 μM SP600125 for 60 minutes and then were stimulated with 2 nM α2AP for 24 hours. The expression of TGF-β in renal fibroblasts were determined by a Western blot analysis. The blots were cropped, and the full-length blots are presented in the supplementary information. The histogram on the right panels shows quantitative representations of TGF-β expression obtained from densitometry analysis (n = 3). The data represent the mean ± SEM. *; P < 0.01. **; P < 0.05.