Fig. 6.

Dynein dissociates from the cortex in cells lacking Myo1. Fluorescence images of Hoechst-stained nucleus (Top and Bottom Left), fluorescent Dhc1 (“Dynein,” Top and Bottom Center), and the intensity map of Dhc1 in wild-type (Top) and Myo1Δ (Bottom) fission yeast zygotes (strain SV111 and strain VA049, respectively; Table S1). The intensity range is shown below the images. The schematics that appear alongside the images depict the localization of dynein at different locations in the cell. In a Myo1Δ background, dynein is absent from the cortex and the microtubules and appears almost exclusively on the SPB. (Scale bar, 2 µm.)