Table S1.
Strains and plasmids used in this study
| Name | Genotype | Source |
| AJC-D40 | h+ leu1-padh1-PHP-GFP-leu1-32 ura4-D18 | John Callegari |
| AY277 | h90 num1-3mCherry-hphR ade6-M216 leu1 ura4-D18 | Akira Yamashita |
| FC1261 | h– myo1(H/TH1)-kanMX leu1-32 ura4-D18 ade6 | Fred Chang |
| FY12794 | h90 leu1-32 ura4-D18 ade6-M210 cam2::ura4+ | NBRP |
| FY13570 | h− myo1::kanr ade6-M216 leu1 | NBRP |
| FY13572 | h90 myo1::kanr ade6-M216 leu1 | NBRP |
| FY13579 | h− myo1+-GFP<<kanr ade6-M210 leu1 | NBRP |
| FY15622 | h90 leu1 lys1 ura4-D18 | NBRP |
| FY16831 | h− ade6-M26 ura4- D18 mcp5::ura4+ | NBRP |
| FY16854 | h+ his2 leu1-32 ura4-D18 mcp5::[mcp5-GFP-3′UTR-Lys3+] | NBRP |
| FY16855 | h− ade6-M2106 mcp5::[mcp5-GFP-3′UTR-Lys3+] | NBRP |
| FY16951 | h90 myo1::GFP cam2::ura4 ura4-D18 | NBRP |
| IF960 | h90 num1-3mCherry-hphR ade6-M216 leu1 | Akira Yamashita |
| KI001 | h+ sid4-GFP::kan r kan r -nmtP3-GFP-atb2+ nmt1-pCOX4-RFP::leu1+ ura4-D18 ade6-M210 | Iva Tolić |
| KP167 | h− leu1 its3-1 | Kuno Takayoshi |
| L972 | h- WT | Iva Tolić |
| L975 | h+ WT | Iva Tolić |
| MMY1824 | h+ pAct1 Lifeact-GFP::leu1+ ade6-210 ura4-D18 leu1-32 | Mithilesh Mishra |
| SV91 | h90 mcp5-mCherry-kan r dhc1-GFP-Leu2 leu1-32 lys1 ura4-D18 | Iva Tolić |
| SV111 | h90 dhc1-mCherry-nat r leu1-32 lys1 ura4-D18 | Iva Tolić |
| VA024 | h90 leu1-padh1-PHP-GFP-leu1-32 ura4-D18 ade6-M26 mcp5::ura4+ | This study |
| VA026 | h90 leu1-padh1-PHP-GFP-leu1-32 ura4-D18 num1-3mCherry-hphR ade6-M216 | This study |
| VA044 | h- myo1::kanr leu1 num1-3mCherry-hphR ade6-M216 ura4-D18 | This study |
| VA045 | h+ myo1::kanr leu1 num1-3mCherry-hphR ade6-M216 ura4-D18 | This study |
| VA046 | h90 myo1+-GFP<<kanr ade6-M210 leu1 num1-3mCherry-hphR ade6-M216 ura4-D18 | This study |
| VA049 | h90 myo1::kanr ade6-M216 dhc1-mCherry-nat r leu1-32 lys1 ura4-D18 | This study |
| VA050 | h90 leu1-padh1-PHP-GFP-leu1-32 ura4-D18 myo1::kanr ade6-M216 leu1 | This study |
| VA051 | h90 mcp5-mCherry-kan r dhc1-GFP-Leu2 leu1-32 lys1 ura4-D18 ade6-M210 cam2::ura4+ | This study |
| VA053 | h90 num1-3mCherry-hphR ade6-M216 leu1 ura4-D18 myo1(H/TH1)kanMX | This study |
| FYP2932 (plasmid) | pRGT1 mcp5-GFP | NBRP |
| FYP2934 (plasmid) | pRGT1 mcp5 (C-CΔ)-GFP | NBRP |