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. 2017 Mar 13;114(13):3473–3478. doi: 10.1073/pnas.1617636114

Fig. 5.

Fig. 5.

KLF4 regulates eRNAs and target genes to control G1/S transition. (A) The KLF4 motif was identified as the most significant one enriched in G0/G1-specific eRNA regions. (B) Example of KLF4 eRNAs (marked with red boxes) near the target gene MLPH. (C and D) KLF4 RNA (C) and protein levels (D) across different cell-cycle stages. Tubulin was used as the loading control for Western blot analysis. (E) Flow cytometry cell-cycle analysis of MCF-7 cells in which KLF4 was silenced with siRNAs. (F) Cell growth analysis of MCF-7 cells upon silencing of KLF4. Mock, without transfection. (G) Validation of candidate KLF4 target genes identified through eRNA analysis. RT-PCR was performed for candidate KLF4 target genes upon silencing of KLF4. Error bars indicate the SEM; *P < 0.05; ** P < 0.01 (t test).