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. 2017 Mar 14;114(13):3539–3544. doi: 10.1073/pnas.1700850114

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Fig. 4. — COP1/SPA inhibits BIN2-mediated PIF3 destabilization. (A) Levels of PIF3-M in Col-0, cop1-4, cop1–4 bin2-3 bil1 bil2, and bin2-3 bil1 bil2. Actin was used as a loading control. (B) Protein gel blot of PIF3-WT-Flag and PIF3-nA4-Flag (PIF3-nA4) in Col-0 and cop1–4, respectively. RPN6 was used as a loading control. PIF3-F, PIF3-Flag. (C) mRNA levels of BIN2-Flag (BIN2-F) in Col-0 and cop1–4. Data shown are representative of two independent lines. (D) Protein levels of BIN2-F in Col-0 and cop1–4. RPN6 was used as a loading control.