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. 2017 Mar 14;114(13):3539–3544. doi: 10.1073/pnas.1700850114

Fig. S5.

Fig. S5.

COP1 interacts with BIN2 in the dark. (A) BIN2 interacts with N-terminal region of COP1 in yeast cells. COP1 (1–675 aa), COP1N (1–127 aa), COP1C (128–675 aa). (B) In vitro pull-down assays showing the interaction between GST-BIN2 and MBP-COP1. (C) Co-IP experiment showing that BIN2-Flag interacts with COP1 in the dark. The anti-Flag conjugated beads were used for the immunoprecipitation experiments with samples of dark-grown Col-0 and 35S:BIN2-Flag /Col-0. Tubulin was used as a loading control.