Fig. 8.

Effect of TOR on LM during PBM period. (A) Nile red staining of FBs was performed on iTOR female mosquitoes and compared with iLuc control samples. LD size increased in iTOR mosquitoes. (B) TAG was measured in iTOR and iLuc mosquitoes. dsTOR treatments were normalized to iLuc. At least three independently collected samples for each treatment were analyzed. (C and D) qRT-PCR results showing significantly reduced mRNA levels of HNF4 (C) and LM (D) genes (VLCAD and 3KCT) in iTOR mosquitoes. Metrics in the y axis in C and D represent relative fold changes. Error bars represent the SEM; *P < 0.05, **P < 0.01.