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. 2017 Mar 14;114(13):E2709–E2718. doi: 10.1073/pnas.1619326114

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Fig. S3. — The influence of JH and Met on representative LM genes and metabolites. (A) The in vivo effect of topical application of JH on LM gene expression. The fold change of LM genes in JH-treated mosquitoes was normalized to those treated with solvent (acetone) only and is indicated on the y axis of each graph. (B) The mRNA levels of LM genes after JH or solvent (acetone) was added to the medium of the in vitro FB culture. Relative fold changes with JH and solvent treatment were normalized to nontreated (NT) samples and are indicated on the y axis of each graph. Error bars represent the SEM; **P < 0.01 in A and B. (C) GC-MS results showing the relative level of C18:1 and C18:2 after Met RNAi knockdown. Error bars represent the SEM; ns, not significant.