Table S1.
Intermolecular cross-linked peptides of the complex formed by holo-PhuS H212R and apo-HemO
| Observed monoisotopic peak* (m/z) | Experimental peptide mass (Da) | Theoretical peptide mass (Da) | Peptide mass matches to intermolecular cross-linked peptides† |
| 2,933.339‡ | 2,932.331‡ | 2,932.425‡ | (PhuS: 1MSSTPSLSHSPAELYRAWQDLR22)^(paHO: 15SQR17)‡ |
| 1,615.886‡ | 1,614.878‡ | 1,614.859‡ | (PhuS: 30AR31)^^^(paHO: 18LNLLTNEPHQR28)‡ |
| 1,615.869 | 1,614.861 | 1,614.863 | (PhuS: 207DTHHFHALLKK217)^^^(paHO: 35SK36) |
| 1,702.013‡ | 1,701.005‡ | 1,700.984‡ | (PhuS: 217KHGAQR222)^(paHO: 124LGAAFLFKK132)‡ |
| 1,875.970 | 1,874.962 | 1,874.906 | (PhuS: 217KHGAQR222)^^^^^(paHO: 1MDTLADESTR10) |
| 1,285.748 | 1,284.740 | 1,284.680 | (PhuS: 217KHGAQR222)^^^^^(paHO: 11QNLR14) |
| 1,133.649 | 1,132.641 | 1,132.585 | (PhuS: 218HGAQR222)^^^(paHO: 11QNLR14) |
| 1,702.855 | 1,701.847 | 1,701.913 | (PhuS: 218HGAQRTQALR227)^^^(paHO: 11QNLR14) |
| 1,726.989 | 1,725.981 | 1,725.913 | (PhuS: 218HGAQRTQALR227)^^^^^(paHO: 11QNLR14) |
| 2,167.239‡ | 2,166.231‡ | 2,166.217‡ | (PhuS: 218HGAQRTQALR227)^^^(paHO: 124LGAAFLFKK132)‡ |
| 1,956.041‡ | 1,955.033‡ | 1,954.961‡ | (PhuS: 218HGAQR222)^(paHO: 133AAALELDENFGAR145)‡ |
| 1,165.672 | 1,164.664 | 1,164.636 | (PhuS: 223TQALR227)^^^^(paHO: 11QNLR14) |
| 1,329.665‡ | 1,328.657‡ | 1,328.684‡ | (PhuS: 223TQALR227)^^^(paHO: 37EPFASR42)‡ |
| 1,303.724‡ | 1,302.716‡ | 1,302.693‡ | (PhuS: 296TTGIAELWR304)^^(paHO: 35SK36)‡ |
*
Peaks of interest were absent in the noncross-linked negative control.
†
Formaldehyde cross-linking and modification are indicated by ^.
‡
Peptides correspond to regions shown to undergo EX1 kinetics.