Fig. 2.

Dual-Glo luciferase reporter assays of long genomic portions of IMPA1.1 (A) and MIPS (B) genes. Black columns represent isosmotic controls (315 mOsmol/kg), and gray columns represent OmB cells dosed in hyperosmotic medium (650 mOsmol/kg). Numbers on the x axis denote the location of genomic sequence fragments relative to the TSS. Double asterisks indicate sequences in which the F/R ratio increased significantly during hyperosmotic stress (P < 0.01; F test followed by t test). One-way ANOVA combined with Tukey's test for assessing statistical significance yielded the same results. Data shown are means ± SD of four biological replicates; RLU, relative luciferase activity units.