Fig. 4.

(Upper) Multiple sequence alignment of six different regions in IMPA1.1 and MIPS genes that match a common consensus, which has OSRE activity. The CRE fitting this consensus motif was named OSRE1. (Lower) The table depicts the results of dual luciferase reporter assays for synthetic constructs representing a core within the OSRE1 consensus that is shared with mammalian TonE/ORE (Row 1) and the most common consensus of OSRE1 in sense (Row 2) and antisense (Row 3) orientation. The right-most column shows the fold change of reporter activity, i.e., the F/R ratio at hyperosmolality compared with the F/R ratio at isosmolality. Data are shown as means ± SD.