Fig. 1.

Analysis approach. Starting from a target amino acid sequence, we use Pfam to extract MSA of homologous sequences and exemplary monomer protein structures from the PDB. DCA is run on the MSA to predict a contact map. Interprotein residue contacts are obtained by pruning all those DCA-predicted residue pairs that are compatible with the known monomeric structure. To obtain a structural model for the biological assembly, we use two monomers (possible homology-modeled if not directly available in the PDB) and dock them guided by the DCA interprotein predictions. BA, biological assembly; CM, contact map.