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. 2017 Mar 10;114(13):E2776–E2785. doi: 10.1073/pnas.1620498114

Fig. 2.

Fig. 2.

Transcriptional profiles of OT-I and P14 TILs and comparison with other exhaustion models. (A) MA plot showing the log2-fold change of mRNA transcript levels and average counts per million (CPM) between OT-I and P14 TILs, isolated from B16-OVA tumors 8 days after in vivo transfer (averaged from three to five biological replicates). Each dot represents an expressed gene. Red and blue dots indicate genes significantly up-regulated [abs(log2 FC) ≥ 1 and FDR ≤ 0.05] in OT-I or P14 TILs, respectively. (B) Heat maps of expression of representative genes up-regulated in OT-I versus P14 TILs, showing individual replicates. (C) Venn diagrams showing the overlap between genes up-regulated in day 8 OT-I versus P14 TILs (red) and genes up-regulated (green) or down-regulated (yellow) in exhausted versus effector CTLs isolated from mice 15 days after LCMV infection (37). The percentage of genes up-regulated in OT-I TILs that are also differentially expressed in the indicated comparison is shown in red. The statistical significance of intersections is from Fisher’s exact tests (n.s. if P > 0.05). (D) Venn diagrams showing the overlap between genes up-regulated in OT-I TILs (red) and genes up-regulated (green) or down-regulated (yellow) in CA-RIT-NFAT1–transduced CTLs versus mock-transduced CTLs (38). (E) Heatmap of log2-fold changes of genes shown in B, in OT-I versus P14 TILs; in exhausted versus effector CTLs from mice infected with LCMV (37); or in CA-RIT-NFAT1–transduced CTLs versus control cells (38).