Fig. 5.

Oligomeric state of ATP synthase in HAP1 and 143B ρ⁺ cells and of vestigial ATP synthase complexes in HAP1-A12 and 143B ρ⁰ cells. Mitoplasts were extracted with various concentrations of digitonin/protein (wt/wt) indicated above each lane. The ATP synthase and vestigial complexes were analyzed by blue native-PAGE, and detected by Western blotting with an antibody against the (A) α- or (B) β-subunit. (A and B) Analysis of, respectively, HAP1 wild-type (WT) and HAP1-A12 cells, and 143B ρ⁺ and ρ⁰ cells. In A, citrate synthase (CS) was used as a loading control. d, dimers; m, monomers; m*, other complexes of unknown oligomeric state; o, oligomers.