Table S1.
Table of statistics detailing processing of tracks
Tracking statistics | 10 μM | 20 μM | 30 μM |
Total time, s | 7,458 | 5,534 | 3,130 |
Threshold for identifying tracking errors, nm | 300 | 550 | 700 |
Total time after removal of poorly tracked episodes, s | 7,358 | 4,979 | 2,684 |
Average no. of tracking errors remaining per track | 2 | 2 | 3 |
No. of slow growth episodes | 2 | 3 | 5 |
Total time after removal of slow growth episodes, s | 7,339 | 4,889 | 2,521 |
% Total time remaining | 98 | 88 | 81 |
Tracking statistics for three experimental conditions: 10 μM, 20 μM, and 30 μM tubulin. Tracking errors were identified as deviations from constant growth above a threshold value. Errors often appeared consecutively and so poorly tracked episodes were removed from the data entirely. The tracking errors remaining per track were then corrected. In the second step slow growth episodes were identified and also removed from the data (SI Materials and Methods and Fig. S2).