Table 4. Enzyme deamination half-life.
Substrate |
t
1/2 (s) |
HPLC-based conversion a (%) | ||
UV-vis | PL | HPLC | ||
A b | 57 | — | — | 100 |
tzA b | 39 | 33 | — | 99 |
2-AA | 207 | — | — | 100 |
tz2-AA | 29 | 28 | 33 | 100 |
A/tzA | — | — | 66/54 | 87/94 |
A/tz2-AA | — | — | 64/59 | 100/100 |
tzA/tz2-AA | — | — | 30/56 | 92/88 |
aHPLC peak area quantification over a 600 s reaction time.
bFrom previous work.9