Table 1.
Effect of the venoms at the IC50 value on the activity of the antioxidant enzymes and MDA level in PC3 cells
| Variable/mg protein | Control cell | PC3 cells treated with | ||
|---|---|---|---|---|
|
| ||||
| Androctonus amoreuxi | Cerastes cerastes | Mix of A. amoreuxi & C. cerastes venoms | ||
| CAT (mU) | 0.84 ± 0.10 | 1.49 ± 0.12 (0.02) | 2.90 ± 0.33 (0.04) | 4.00 ± 0.27 (0.003) |
| SOD (U) | 168.75 ± 0.81 | 232.20 ± 9.01 (0.03) | 385.75 ± 32.78 (0.03) | 638.24 ± 41.81 (0.01) |
| GPx (mU) | 4.60 ± 0.31 | 7.64 ± 0.48 (0.02) | 14.05 ± 0.49 (< 0.001) | 13.03 ± 0.38 (0.0002) |
| GR (mU) | 17.82 ± 0.43 | 27.73 ± 0.79 (0.003) | 47.29 ± 4.01 (0.03) | 86.27 ± 7.34 (0.02) |
| GST (mU) | 3.09 ± 0.10 | 5.22 ± 0.26 (< 0.01) | 8.97 ± 0.29 (0.004) | 9.68 ± 0.24 (0.0002) |
| MDA level (nmol) | 4.43 ± 0.20 | 6.77 ± 0.49 (0.04) | 14.17 ± 0.90 (0.01) | 16.17 ± 1.13 (0.01) |
Values are present as mean ± SE (P value). PC3 cells were treated with the venoms for 24 hours. MDA, malondialdehyde; PC3, prostate cancer cell line; CAT, catalase; SOD, superoxide dismutase; GPx, glutathione peroxidase; GR, glutathione reductase, GST, glutathione-S-transferase.